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| Potrich,Michele; Silva,Rita T.L. da; Maia,Fabiana M.C.; Lozano,Everton R.; Rossi,Robson M.; Colombo,Fernanda C.; Tedesco,Flavia G.; Gouvea,Alfredo de. |
| ABSTRACT This study aimed to evaluate the effect of commercially used entomopathogens on Africanized Apis mellifera L. (Hymenoptera: Apidae). Four bioassays were performed: 1) pulverized entomopathogens on A. mellifera; 2) entomopathogens sprayed on a smooth surface; 3) entomopathogens sprayed on soy leaves; and 4) entomopathogens mixed with candy paste (sugar syrup). Five treatments were prepared: sterile distilled water (control), distilled water sterilized with Tween® 80 (0.01%), and the commercial entomopathogens Metarhizium anisopliae E9 (1.0 × 109 conidia mL−1), Beauveria bassiana PL63 (1.0 × 108 conidia mL−1) and Bacillus thuringiensis var. kurstaki HD-1 (3.0 × 108 spores mL−1). Each treatment consisted of five repetitions, with 20 workers per... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bayesian statistics; Entomopathogenic fungi; Entomopathogenic bacteria; Honeybee; Selectivity. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262018000100023 |
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| Zorzetti, Janaina; Ricietto, Ana Paula Scaramal; Fazion, Fernanda Aparecida Pires; Meneguim, Ana Maria; Neves, Pedro Manuel Oliveira Janeiro; Vilas-Bôas, Gislayne Trindade. |
| Elasmopalpus lignosellus (Zeller, 1848) (Lepidoptera, Pyralidae) is an insect pest of 60 economically important crops, including sugarcane, wheat, soybean, rice, beans, sorghum, peanuts, and cotton. The aim of this work was to select and characterize Bacillus thuringiensis isolates with insecticidal activity against E. Lignosellus that could be used as an alternative method of control. Selective bioassays were done to evaluate the toxicity of 47 isolates against first instar larvae of E. lignosellus. For the most toxic bacterial strains, the lethal concentration (LC50) was estimated and morphological, biochemical and molecular methods were used to characterize the isolates. Among the 47 isolates tested, 12 caused mortality above 85% and showed LC50 values... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agronomia; Fitossanidade biological control; Cry genes; Entomopathogenic bacteria; Lesser cornstalk borer. Controle microbiano de pragas. |
| Ano: 2017 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/32707 |
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| Zorzetti,Janaina; Ricietto,Ana Paula Scaramal; Fazion,Fernanda Aparecida Pires; Meneghin,Ana Maria; Neves,Pedro Manuel Oliveira Janeiro; Vilas-Boas,Laurival Antonio; Vilas-Bôas,Gislayne Trindade. |
| ABSTRACT The coffee berry borer Hypothenemus hampei Ferrari, 1876 (Coleoptera: Curculionidae: Scolytinae) is considered the most serious pest of the coffee crop and is controlled primarily with the use of chemical insecticides. An alternative to this control method is the use of the entomopathogenic bacterium, Bacillus thuringiensis Berliner, 1911. Therefore, the objective of this work was to select strains of B. thuringiensis virulent against H. hampei and characterize them by morphological and molecular methods to identify possible genes for the production of genetically modified plants. To achieve this objective, 34 strains of B. thuringiensis underwent a selective bioassay to evaluate their toxicity to H. hampei first-instar larvae. Among the strains... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological control; Entomopathogenic bacteria; Coffee berry borer; Cry proteins. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262018000300198 |
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| Berlitz,Diouneia Lisiane; Azambuja,Aline Oliboni de; Sebben,Alessandra; Oliveira,Jaime Vargas de; Fiuza,Lidia Mariana. |
| Oryzophagus oryzae (Costa Lima 1936) (Coleoptera: Curculionidae) and Spodoptera frugiperda (J E Smith, 1797) (Lepidoptera: Noctuidae) cause important crop losses in southern Brazil. Control is possible by the use of the bacteria Bacillus thuringiensis and extracts of Melia azedarach. This study aimed to evaluate the mortality, in vivo, of O. oryzae and S. frugiperda submitted to two isolates of B. thuringiensis and the aqueous extract of M. azedarach. The LC50 for O. oryzae due to bacteria was 5.40μg/mL (Bt 2014-2) and due to plant extract 0.90μg/mL. For S. frugiperda, the Bt 1958-2 bacterial suspension (1.10(10)UFC/mL) caused a 100% of corrected mortality, showing that the purified Cry proteins caused a CL10 of 268μg/mL five days after the treatments, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alternative pest control; Entomopathogenic bacteria; Insecticidal activity; Botanical insecticidal. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500012 |
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| Castilhos-Fortes,Raquel de; Matsumura,Aida T.S.; Diehl,Elena; Fiuza,Lidia M.. |
| The effects of Bacillus thuringiensis (Bt) Berliner on the termite Nasutitermes ehrhardti (Isoptera, Termitidae) were evaluated under laboratory conditions. From 55 Bt subspecies assayed in vivo under controlled conditions seven were found to be pathogenic in the subspecies yunnanensis, huazhongiensis, brasiliensis, colmeri and kurstaki (less than 72% of mortality), particularly sooncheon and roskildiensis (100% mortality at the seventh day after the bacteria application). The LC50 for subspecie sooncheon corresponded to 47x10(8),()66.2x10(6) and 5.1x10(5) cells/ml, at the third, fifth and seventh day, respectively. For the subspecie roskildiensis the LC50 corresponded to 30.8x10(5), 48.4x10(6) and 16.8x10(4) cells/ml, at the third, fifth and seventh day,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus thuringiensis; Biological control; Entomopathogenic bacteria; Nasutitermes ehrhardti; Termite. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300006 |
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